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Description
MSP1D1E3 His Tag Protein, Human (Nanodisc)Product Specification Species Human Synonyms MSP1D1E3 Concentration 1. 8mg ml by Nanodrop Amino Acid Sequence Target(266 amino acid) with N terminal 6x His and Factor Xa site Expression System E. coli Molecular Weight 32. 68 kDa Purity > 90% as determined by SDS PAGE & SEC HPLC. Conjugation Unconjugated Tag His Tag Storage Buffer 40 mM Tris, ph8. 0, 300 mM NaCl. Stability & Storage 6 months from date of receipt, 60 to 80 C as supplied. about 3 days in
Product Specification
| Species | Human |
| Synonyms | MSP1D1E3 |
| Concentration | 1.8mg/ml by Nanodrop |
| Amino Acid Sequence | Target(266 amino acid) with N terminal 6x His and Factor Xa site |
| Expression System | E.coli |
| Molecular Weight | 32.68 kDa |
| Purity | > 90% as determined by SDS-PAGE & SEC-HPLC. |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Storage Buffer | 40 mM Tris, ph8.0, 300 mM NaCl. |
| Stability & Storage | ·6 months from date of receipt, -60 to -80 °C as supplied. |
| Reference | 1. Grinkova Y V, Denisov I G, Sligar S G. Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers[J]. Protein engineering, design & selection, 2010, 23(11): 843-848. |
Background
The soluble lipid bilayer nanodisc has proven to be a widely applicable method for membrane proteins as soluble in aqueous solutions, which can monodisperse and active membrane protein in a closely native bilayer environment. The key component of the nanodisc is the surrounding amphiphilic spiral protein band (membrane scaffold protein). Nanodisc systems have been used to reconstruction a variety of proteins including GPCRs, P450, bacterial rhodopsin, clotting factors, and tar receptors. Membrane scaffold protein 1E3 with larger inner diameter for twelve transmembrane membrane protein has been used as a scaffold protein for stabilizing lipid nanodiscs (ND). Membrane scaffold protein 1E3 (MSP1D1E3) is modified versions of apolipoprotein A1. It is a biparental synthetic protein that can self-assemble to form nanodiscs. The diameter of MSP1D1E3 is about 12 nm.
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